Journal: Nucleic Acids Research

Article Title: DNA sequence templates adjacent nucleosome and ORC sites at gene amplification origins in Drosophila

doi: 10.1093/nar/gkv766

Figure Lengend Snippet: Nucleosome position at DAFC-66D is largely determined by primary DNA sequence. ( A ) Comparison of observed nucleosome occupancy at DAFC-66D in follicle cells in stage 10 (dark blue) with predicted nucleosome occupancy (light blue) based on nucleosome DNA sequence preferences ( , ). Red asterisks indicate three predicted nucleosome positions that were less occupied in vivo than predicted. ( B and C ) Expanded view of predicted and observed nucleosome occupancy at ACE3 ( B ) and Ori-β ( C ), with nucleotide composition plotted above (see color key). Nucleosome occupied sites are relatively GC rich (black and blue), while nucleosome depleted regions in ACE3 and Ori-β contain extended poly A:T tracts (red and green) that correspond to ORC binding sites.

Article Snippet: Nucleotide composition at DAFC-66D was determined over a sliding 50 bp window using the nucleotide composition analysis software of MacVector (version 13.5.2) ( ).

Techniques: Sequencing, Comparison, In Vivo, Binding Assay